Coding

Part:BBa_K128003:Design

Designed by: Sara Mouradian   Group: iGEM08_MIT   (2008-07-11)


p1025


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 81
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 82
    Illegal PstI site found at 96
    Illegal NotI site found at 7
    Illegal NotI site found at 89
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 82
    Illegal PstI site found at 96
    Illegal NgoMIV site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible.


Source

This peptide is a 20 amino acid section of the native S.mutans surface protein that binds to the glycoproteins of the teeth. It is taken straight from the 1999 Nature paper by CG Kelly et al. which discovered the efficacy of this peptide in competitive inhibition of S.mutans.

References